With the development of industry and the improvement of living standards, better quality in power electric service is required more than ever before. This paper deals with the optimal algorithms for voltage regulation in the case where Distributed Storage and Generation (DSG) systems are operated in distribution systems. It is very difficult to handle the interconnection issues for proper voltage managements, because the randomness of the load variations and the irregular operation of DSG should be considered. This paper proposes the optimal on-line real time voltage regulation methods in power distribution systems interconnected with the DSG systems. In order to deliver suitable voltage to as many customers as possible, the optimal sending voltage should be decided by the effective voltage regulation method by using artificial neural networks to consider the rapid load variation and random operation characteristics of DSG systems. The simulation results from a case study show that the proposed method can be a practical tool for the voltage regulation in distribution systems including many DSG systems.