The purpose of this study was to investigate the seroprevalence of paratuberculosis in Korean cattle in western Jeonbuk area. From February 2012 to January 2013, blood samples were collected from 2,606 Korean cattle of 263 farms. As a result, 60 (2.3%) heads of 46 (17.5%) farms were positive on the ELISA test for paratuberculosis. Based on regional analysis, 18 (19.6%) out of 92 farms and 24 (2.5%) out of 941 heads in Iksan area, 28 (22.0%) out of 127 farms and 36 (2.8%) out of 1,291 heads in Kimje area were positive but samples from Gunsan area were all negative. According to scale breeding, small scale (below 50 heads) breeding showed the most high prevalence rate compared to middle (50 to 99 heads) or large (over 100 heads) scale breeding. To clarify the relation between number of heads and paratuberculosis prevalence, some additional analysis would be required in further, though.