This study investigated the effect of whole-body vibration on muscle function and muscular reaction in the knee joint. We recruited thirty healthy subjects and divided them into a training group, who experienced whole-body vibration, and a control group, who did not. The training group performed whole-body vibration exercises for 30 min per day, 3 days a week, for 8 weeks. We measured knee joint torque to estimate muscle strength and reaction, using BIODEX System 3. Knee joint peak torque and total work performed increased significantly in the training group, and muscle acceleration time decreased. These results suggest that stimulation by whole-body vibration can improve muscle strength and reaction by improving muscle tone and increasing blood temperature and flow speed in muscular fiber. Our results also indicate that 4 weeks of exercise with whole-body vibration is required to improve the reaction response, and six weeks to improve strength.