This study is to evaluate the effects of the separation wall on the sediment quality and quantity in a combined sewer, by surveying the sewer overflow and sediments during a rainfall. Since the separation wall installed in the combined sewer separates the rainfall and the sewage, the flow rate of the sewage is increased, and the amount of the sediment deposited on the sewer is decreased. One sampling point was the outfall of Daesacheon with a separation wall, and the other was the outfall of Gwaryecheon without a separation wall, in Daejeon metropolitan city. The maximum control of the biochemical oxygen demand (BOD) overflow load was more than 38% in the Daesacheon point with the separation wall, during a rainfall of 0.11 mm/hr. The maximum control of the BOD overflow load was 24% in Gwaryecheon without a separation wall, during a rainfall of 1.0 mm/hr. According to the survey results of the sediment in the sewer, the discharged sediment deposited on the sewer in Gwaryecheon point was about 23% to 28% of the total suspended solid during the rainfall. In addition, the average velocity of sewage in the presence of sediment was about 0.30 m/s, and if the separation wall is installed, it was expected to be about 1.01 m/s, that is 3.4 times more than the same conditions, resulting in the reduction of the sediment deposit.