Rhodamine dyes have been studied in various scientific areas due to their excellent photophysical properties. In particular, these rhodamine dyes are one of the most famous fluorophores as signal unit in chemosensor study. This is related to spirolactam ring system in rhodamine dyes. When the spirolactam ring is closed, there is nonfluorescence and colorless. Whereas, ring-opening of the corresponding spirolactam induces strong fluorescence and color. These absorption and emission changes are related to structural changes as well as electron energy potential levels such as HOMO and LUMO values. In this study, two different structures of rhodamine 6G hydrazide depending on the spirolactam ring system were investigated using absorption measurement, electrochemical measurement and computational calculations.