This study examined cross-generational changes in the vowel system of Seoul Korean. Acoustic analyses of the vowel formants of /o/ and /u/, and their Euclidean distances in the vowel space were undertaken to explore an on-going merger of these two vowels as proposed in previous acoustic studies and a phonological analysis by Chae (1999). A robust cross-generational change of /o/ and /u/ was found, more evident for female speakers than for male speakers. For female speakers, with each successive generation, /o/ became increasingly approximated with /u/, regardless of the syllable positions that the target vowels were posited, whereas the cross-generational differences in the Euclidean distances were only shown in the second syllable position for the male speakers. These results demonstrate that 1) women are more advanced than men in the on-going approximation of /o/ and /u/; 2) the approximation of /o/ and /u/ is common in the non-initial position. Taken together, the merger of /o/ and /u/ appears to be in progress in Seoul Korean.