In this study, focusing of ultrasonic Lamb wave by negative refraction with mode conversion from antisymmetric to symmetric mode was investigated. When a wave propagates backward by negative refraction, the energy flux is antiparallel to the phase velocity. Backward propagation of Lamb wave is quite well known, but the behavior of backward Lamb wave at an interface has rarely been investigated. A pin-type transducer is used to detect Lamb wave propagating on a steel plate with a step change in thickness. Conversion from forward to backward propagating mode leads to negative refraction and thus wave focusing. By comparing the amplitudes of received Lamb waves at a specific frequency measured at different distance between transmitter and interface, the focusing of Lamb wave due to negative refraction was confirmed.