A greenhouse soil infested with an obligate nematode parasitic bacterium, Pasteuria penetrans, was used to test the effect of temperatures on the endospore attachment to root-knot nematode, Meloidogyne arenaria, juveniles (J2). Freshly hatched J2s were inoculated to the soil in petri dish and incubated under different temperatures of $20^{circ}C$, $25^{circ}C$, $30^{circ}C$, and $35^{circ}C$ for 7 days. The endospore attachment rates were 100% in all the temperatures, while the number of endospores attached per J2 was highest in $25^{circ}C$ with 28.3 endospores/J2 followed by 20.2, 18.6, and 13.6 in $30^{circ}C$, $20^{circ}C$, and $35^{circ}C$, respectively. When the soil was pre-treated under different temperatures before the J2 inoculation, the endospore attachment rates significantly decreased from 60% in room temperature to $25^{circ}C$, 31.7, 8.3, 5.0, and 0% after the soil incubation in $-30^{circ}C$, $4^{circ}C$, $40^{circ}C$, $50^{circ}C$, and $100^{circ}C$ for 10 days, respectively. The endospore numbers attached per J2 were 3.5, 4.3, 1, 1, and 0 when the soil was pre-treated in $-30^{circ}C$, $4^{circ}C$, $40^{circ}C$, $50^{circ}C$, and $100^{circ}C$, respectively, which were lower than 5.3/J2 of room temperature treated soil. The P. penetrans isolate in the soil showed nematode species-specific endospore attachment characteristics with 100% attachment rate only on M. arenaria J2s while the rates were 0% on M. hapla and M. incognita J2s.