This study had collected 267 Dental Health Care Worker in Busan, Gyeongnam and Ulsan, in order to investigate work-family conflict, job satisfaction and turnover intention of dental hygienists and find relationship between these. A survey was conducted from April 8 through April 30, 2013, by using structured, self-administered questionnaires. The collected data were analyzed with SPSS 12.0 program. The obtained results were as follows; 1. The average job satisfaction point are 3.32, work-family conflict point 3.06, and turnover intention point 2.80. 2. The job satisfaction was under the greatest influence of the Group of Rationality, followed by declination of Work Demand, Working Environment, Money. 3. The turnover intention was under the greatest influence of the Money, Job of Future. All the foctors had a positive impact on their turnover intention. That lowering these factors expressed a positive correlation, the higher the money and job of Future turnover intention.