The fish fauna and disturbance of geographical distributions in the eastern Civilian Control Line of Korea were investigated from May to September 2012. Total 35 species sampled during the period were belonged to 13 families. There were 9 Korean endemic species (25.71%) that distributed in the flows into West and South Sea. Distributions of invasion fishes in the studied streams based on geographical distributions of freshwater fish were total 13 species that classified 1 species, C. cuvieri from foreign country and 12 species from other water systems. Hence there have been anthropogenically introduced from too many years ago. Also groups according to the similarities of each studied stations was divided into 4 groups by similarity 50% because difference in them was considered to effect of domestic invasion species. In conclusion, three streams in the eastern Civilian Control Line of Korea were invaded by many alien fishes. Therefore, we are considered to require precise investigation and monitoring for the preparations to management measure.