The organic Rankine Cycle (ORC) uses a kind of refrigerant as a working fluid that evaporates at relatively low temperature, as the Rankine Cycle uses superheated steam as the working fluid. A small scale ORC test bench was installed, and two different working fluids (R245fa and R134a) were injected into the test bench. The test bench was in operation with the two different working fluids under the same conditions. The effects against the system performance from the different working fluids were analysed, and root causes were identified. Other factors reflecting the power generation efficiency were also found. A conclusion was drawn, that R245fa makes the system perform better, than R134a.