In this paper, microstrip antenna to enhance the isolation between transmitting port and receiving port under the proximity objects is proposed, and applied to the Doppler radar sensor working at 5.8GHz ISM band which detects vital signals of a human body. Two 3dB quadrature hybrids are placed around radiation patch to form a balanced structure between transmitting port and receiving port, such that it consistently provides enhanced Tx/Rx isolation and excellent return loss over nearby objects. It is theoretically analyzed and simulated to verify the validity of the proposed application. The fabricated antenna that is 2mm away from the human body, has more than 16 dB return loss and at least 30dB isolation over ISM frequency band of 5.8GHz.