This study was evaluated to biological activity of breeding lines from Acantopanax( A. sessiliflorus: ASF, A. koreanum: AKN, A. chiicanensis: ACS, A. senticosus: AST) and parts(root, stem, fruit, and leaf) and various extracted solvents( 100% water, 100% EtOH, 50% EtOH). Total polyphenol content of AKN root in 100% water extracts was high detected 464.46 mg/100 g. Total flavonoid content in the leaf was significantly higher than in other parts. The content of total sugars was high in the 50% EtOH extracts and fruit. The major free amino acids were arginine in all extracts. The content of arginine was detected in the root of AKN(1.807 mg/100 mg). Contents of eleutheroside B, E were high detected in 100% water extracts. Antioxidative capacity in the leaves of AKN was the higher than other extracts($EC_{50}=84.8{mu}g/mL$). The results would be useful for understanding of the physiological properties of AKN extracts.