The purpose of the study was to investigate the effects of gymnasts shoes on risk factors of anterior cruciate ligament injuries during drop-landing followed by vertical jump in female gymnasts. Thirteen female gymnasts were recruited and performed randomly drop-landing followed by vertical jump in height of her knee with and without shoes. Kinematics and ground reaction data were collected to estimate the anterior cruciate ligament injuries risk factors. Data were analyzed with paired samples t-test with Bonferroni correction. Female gymnasts with shoes showed more reduced thigh maximum adduction angle, and knee maximum extension moment than without shoes. Female gymnasts with shoes showed more increased shank maximum abduction angle than without shoes. In conclusion, Female gymnasts with shoes reduced anterior cruciate ligament injuries risk factors.