In this study, a dynamic camera actuation system for simultaneous in situ image acquisition is developed to achieve real-time observation of transient liquid flow on a lab-on-a-disk. A disk-type electric circuit, namely circuit-on-a-disk, co-rotated with the lab-on-a-disk improves the dynamic image acquisition ability in terms of a frame rate. The circuit-on-a-disk is comprised of a camera connected with a motor, a microprocessor and a wireless communication module. The camera connected with the motor enables to realize dynamic tracking of a transient flow and real-time image acquisition. The obtained images can be simultaneously transferred by a video/audio transmitter unit to a personal computer. Also, the microprocessor receives signals from the personal computer, and then controls the focusing position of the camera. We are expecting that heaters, sensors, and light sources also can be integrated on the circuit-on-a-disk, and they will enable various functional actuations as well as precise image acquisition.