In this paper, the structural design of optical Internet is analyzed and by using UNI (User Network Interface) signaling protocol an optical transmission experiment was performed. The hierarchical structure of the basic optical Internet consists of the backbone network, the service network and the access network. The necessary functions for each layer were described as follows: Control structure of the optical transport layer, network operation and management structure, internetworking technology of sub networks, routing and signaling technology. By using UNI signaling protocol from OIF (Optical Internetworking Forum), the optical transmission in the proposed structure of the optical Internet network was experimented. By the traffic generation of LSP (Label Switched Path) data packets along the route-configuration was delivered to UNI. Finally, by showing the value of TCP (Transmission Control Protocol) packets the optical transmission was completely and successfully demonstrated.