River otter(Lutra lutra) are listed as endangered species and Natural monument in Korea, and this study examined the possibility of extinct of river otter in Hongchon river using with the application of Population Viability Analysis (PVA) technique. In Hongchon river areas population was estimated 9 individuals for the last 1999-2005 years and PVA analysis was done for the next 10 years using the average population of 9. Using the initial population the river otter was estimated 30% of extinct for the next 10 years. This estimation was quite low considering water pollution and construction of highways. Also PVA only used population size lacking in other life history information. Nonetheless river otter population can be in risk of extinction if the current construction of crossovers, cement bank are maintained. Long term information regarding life history needs essential.