Formulations of the rutin nanosuspensions have been prepared by high pressure homogenization (HPH). A Micron LAB 40 was used for HPH to obtain rutin nanosuspensions. Photon correlation spectroscopy (PCS) and laser diffraction were employed to analyze the particle size. Morphology of the particles was analyzed by light microscopy. The HPH technique produced rutin nanosuspensions having PCS size average of 547-912 nm and zeta potential range about -30 mV in water. Aqueous rutin nanosuspension stabilized by SDS and Tween 80 were stable over 12 months. The nanosuspensions produced via HPH not only could prevent large particle size and particle growth, but also protect the drugs from chemically degradation. The molecules of the surface stabilizer are able to shield the chemical compound. The crystalline structure in a nanoparticulate sized formulation results in improved drug chemical-stability.