Fire resistance and impact sound insulation tests were performed for a floor assembly, of which stiffness was reinforced by shortening the span of floor joists by installing glulam beam additionally in the middle or one thirds of the original span, and which an additional ceiling component was installed apart from floor structure. By applying the isolated ceiling, timber framed floor showed 1 hour of fire resistance even in case that dead load was increased by considering cement mortar layer for radiant floor heating. Insulation performance against light and heavy impact sound was improved significantly by applying the sound absorbing layer of big mass and high elasticity in addition to the stiffness improvement and isolated ceiling.