A total of 24 juvenile specimens of Thunnus tonggol (5.45~7.26mm in total length) of the Sombridae were collected from the southeast sea of Jeju Island during 26~30 July, 2013. Twenty-four specimens identified T. tonggol have melanophores distributed on the 1st dorsal-fin rays, the dorsal of head and vetral side. Three individulas were identified using mitochondrial DNA cytochrome oxidase submit 1 (CO1) sequences (452 base pairs). All were identified as T. tonggol, their mtCO1 sequences being consistent with those of Thunnus tonggol (d=0.000), followed by Thunnus albacares (d=0.002) and Thunnus obesus (d=0.007).