This study was carried out to provide a valuable reference which could reduce heating loss of air-inflated double PO film. Therefore, this study was aimed to choose the best air space thickness and injected air temperature. The characteristics of heat transmission variation at experimental materials were measured and analysed in the laboratory. The experiment was conducted of two layers of PO film, each 0.15 mm tick, sandwiching 110, 175, 225 mm of inflated air with 1 m sides. Environmental control lab was constantly controlled with $-10^{circ}C$ and experiment chamber was constantly changed with 0, 5, 10, $15^{circ}C$. The analysis of heat transfer showed that heat transmission does not have a direct correlation with Air Space thickness and injected air temperature. But when inside and outside temperature difference of chamber was great, supply of outside air to Air Space had an advantage at reduction of heating load. It was required to examine accurate analysis at a real greenhouse.