Recently, Ethernet-based Diagnostic Over Internet Protocol (DoIP) was applied to automotive systems, and in-vehicle gateways have been introduced to integrate Ethernet with traditional in-vehicle networks, such as the local interconnect network (LIN), controller area network (CAN) and FlexRay. The introduction of in-vehicle gateways and of Ethernet based diagnostic protocols not only decreases the complexity of the networks, but also reduces the update time for ECU software reprogramming while enabling the use of a range of services, including remote diagnostics. In this paper, a diagnostic gateway was implement for an automotive system, and the performance measurements are presented. In addition, a range of applications provided by the diagnostic gateway are proposed.