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$small{D}$-Lactic Acid Production by Sporolactobacillus inulinus Y2-8 Immobilized in Fibrous Bed Bioreactor Using Corn Flour Hydrolyzate
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  • $small{D}$-Lactic Acid Production by Sporolactobacillus inulinus Y2-8 Immobilized in Fibrous Bed Bioreactor Using Corn Flour Hydrolyzate
  • $small{D}$-Lactic Acid Production by Sporolactobacillus inulinus Y2-8 Immobilized in Fibrous Bed Bioreactor Using Corn Flour Hydrolyzate
저자명
Zhao. Ting,Liu. Dong,Ren. Hengfei,Shi. Xinchi,Zhao. Nan,Chen. Yong,Ying. Hanjie
간행물명
Journal of microbiology and biotechnology
권/호정보
2014년|24권 12호|pp.1664-1672 (9 pages)
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한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

In this study, a fibrous bed bioreactor (FBB) was used for $small{D}$-lactic acid ($small{D}$-LA) production by Sporolactobacillus inulinus Y2-8. Corn flour hydrolyzed with ${alpha}$-amylase and saccharifying enzyme was used as a cost-efficient and nutrient-rich substrate for $small{D}$-LA production. A maximal starch conversion rate of 93.78% was obtained. The optimum pH for $small{D}$-LA production was determined to be 6.5. Ammonia water was determined to be an ideal neutralizing agent, which improved the $small{D}$-LA production and purification processes. Batch fermentation and fed-batch fermentation, with both free cells and immobilized cells, were compared to highlight the advantages of FBB fermentation. In batch mode, the $small{D}$-LA production rate of FBB fermentation was 1.62 g/l/h, which was 37.29% higher than that of free-cell fermentation, and the $small{D}$-LA optical purities of the two fermentation methods were above 99.00%. In fe$small{D}$-batch mode, the maximum $small{D}$-LA concentration attained by FBB fermentation was 218.8 g/l, which was 37.67% higher than that of free-cell fermentation. Repeate$small{D}$-batch fermentation was performed to determine the long-term performance of the FBB system, and the data indicated that the average $small{D}$-LA production rate was 1.62 g/l/h and the average yield was 0.98 g/g. Thus, hydrolyzed corn flour fermented by S. inulinus Y2-8 in a FBB may be used for improving $small{D}$-LA fermentation by using ammonia water as the neutralizing agent.