Number of components and payload systems installed in satellites were found to be exposed to various disturbance sources such as the reaction wheel assembly, the control moment gyro, coolers, and others. A micro-level of vibration can introduce jitter problems into an optical payload system and cause significant degradation of the image quality. Moreover, the prediction of on-orbit vibration effects on the performance of optical payloads during the development process is always important. However, analyzing interactions between subsystems and predicting the vibration level of the payloads is extremely difficult. Therefore, this paper describes the analytical and experimental approach to microvibration effects on satellite optical payload performance with integrated jitter analysis frame-work, microvibration emulator and satellite structure testbed.