In this study, using a complex of physical, chemical, and biological evaluation factors, the ecological vulnerability to climate change were evaluated at each river in the Nakdong river basin. First, runoff, sediment rate, and low flow discharge changes according to AIB climate change scenario using the SWAT model were simulated. Also, for the assessment of chemical and biological factors, 48 points that water quality monitoring sites and ecological health measurement points are matched with each other was selected. The water quality data of BOD and T-P and the biological data of IBI and KSI in each point were reflected in the assessment. Also, the future rise in water temperature of the rivers in Nakdong river basin was predicted, and the impact of water temperature rise on the fish habitat was evaluated. The top 10 most vulnerable points was presented through a summary of each evaluation factor. This study has a contribution to river restoration or management plan according to the characteristics of each river.