This study was conducted to determine the effects of seed storage temperature and pre-treatment on seed germination, seedling quality and vigor index of wild Trichosanthes kirilowii and Trichosanthes kirilowii var. japonica. As a result the highest germination rate of T. kirilowii was the 95% at seed stored in room temperature and then soaking for 24 hours in $dH_2O$. And the highest germination rate of T. kirilowii var. japonica was 96% at seed stored in $2^{circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). But the seed germination rate was non-significance in pre-treatment at seed stored in room temperature $2^{circ}C$. Seedlings of T. kirilowii and T. kirilowii var. japonica showed not only the best seedling quality but also seedling vigor index in seed stored at $2^{circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). In this research, T. kirilowii and T. kirilowii var. japonica seed were stored in room temperature or $2^{circ}C$, and then sown in peat moss, seed germination rate was more than 90% and production of superior quality seedlings.