Objectives : The purpose of this study was to investigate cognition on oral health education and dental caries preventive effect in kindergartens and child care facilities. Methods : A self-reported questionnaire was completed by 260 teachers in kindergartens and nursery facility in Daejeon from January to February, 2013. Except incomplete answers, 248 questionnaires were analyzed by using the statistical package SPSS WIN 18.0. Results : High intention to participate in oral health education was shown in the teachers who experienced the oral health education. Dental caries preventive effect included correct tooth brushing, fluoride dentifrice, fluoride mouth rinse, and use of xylitol. The teachers agreed that the fluoride mouth rinses would help good oral health in the children. Conclusions : Teachers in kindergartens and nursery facility are the most important persons in childhood oral health care. So it is necessary to provide the continuing standardized education for the teachers systematically.