Objectives : The purpose of the study is to investigate and compare the knowledge of hepatitis B in nursing and dental hygiene students in Busan. Methods : A self-reported questionnaire was filled out by 361 students in nursing and dental hygiene departments in Busan from August 25 to September 4, 2013. Data were analyzed by SPSS version 13.0 for descriptive statistical analysis, ${chi}^2$-test, and t-test and set at p<0.05. Results : Knowledge in hepatitis B consisted of 25 questions including severity of hepatitis B, general knowledge, infection routes, and symptoms and treatment. There was no significant difference between nursing and dental hygiene students in knowledge; nursing students showed an average of $18.21{pm}2.809$ and dental hygiene showed $17.71{pm}3.23$. The average score was $17.96{pm}3.03$ out of 25 questions. Conclusions : There is no significant difference between nursing and dental hygiene students in knowledge of hepatitis B. It is necessary to emphasize the education of hepatitis B periodically in addition to regular curricula.