Objectives: This pilot study assessed secondhand smoke (SHS) exposure in smoking and non-smoking nightclubs in Seoul, Korea by measuring the concentration of particulate matter smaller than $2.5{mu}m$ ($PM_{2.5}$). Methods: This comparative study was conducted in three nightclubs in Seoul. While one non-smoking nightclub was measured on weekdays and weekends, different smoking nightclubs were measured on weekdays and weekends. The concentration of $PM_{2.5}$ was observed using a real-time monitor over an average of three hours. The number of people in the clubs was also estimated. Settled dust was collected in a smoking and a non-smoking nightclub and analyzed for NNK concentration. Results: The $PM_{2.5}$ concentration in the smoking nightclubs was higher than those found in the non-smoking nightclub by 26 times on weekdays and three times on weekends. Indoor $PM_{2.5}$ concentration was correlated with the number of people in the smoking nightclubs. Relatively high $PM_{2.5}$ concentration was observed in the non-smoking nightclub on weekends. NNK concentration in the smoking nightclub was 7 times higer than in the non-smoking nightclub. Conclusion: Smoking in nightclubs caused high $PM_{2.5}$ concentration. Although the non-smoking nightclub had a lower $PM_{2.5}$ concentration, $PM_{2.5}$ concentration on weekends was higher due to the smoking room. Complete prohibition of smoking in nightclubs can protect patrons from secondhand smoke exposure.