A parametric study on anti-vortex holes for turbine blade cooling was investigated numerically. Three-dimensional Reynolds-averaged Navier-Stokes equations and shear stress transport turbulence model were used for analysis of anti-vortex film cooling. Validation of numerical results was carried out comparing with experimental data. The cooling performance of anti-vortex holes was assessed by two geometric variables, the ratio of diameters of holes and the lateral distances between the primary hole and anti-vortex hole at blowing ratios of 0.5 and 1.0. The results showed that the spatially-averaged film-cooling effectiveness increases as the ratio of the diameters increases and the distance between the primary hole and anti-vortex hole decreases.