A low-noise regenerative blower is developed for fuel cell application by combining the FANDAS-Regen code and design optimization algorithm under several performance constraints for flow capacity, static pressure, efficiency and power consumption. The optimized blower design model is manufactured with some impeller modification based on low noise design concept and tested by using aerodynamic performance chamber facility and narrow-band noise measurement apparatus. The measured results of the optimized blower satisfy the performance requirements and are also compared favorably with the FANDAS-Regen prediction results within a few percent relative error. Furthermore, the present study shows the remarkable noise reduction by 26 dBA can be achieved through design optimization and low noise design concept.