Purpose : The purpose of this study was to perform the calf-raise exercise on the stable support surface and unstable support surface for comparing deference of the gastrocnemius thickness changes that measured by ultrasonography. Method : Subjects stand on unstable support surface and a stable support surface with both feet. Keeping calf-raise position in the 3 seconds, and then had to return back to the starting position. Total of 3 sets of 15 repetitions performed and give a 30-second rest between each set. In this way, exercise performed 3 times a week, performed a total of 6 weeks. Result : 1. gastrocnemius muscle thickness before and after exercise in experimental group A was significantly different(p<.05). 2. gastrocnemius muscle thickness before and after exercise in experimental group B was significantly different(p<.05). 3. gastrocnemius muscle thickness in experimental group A and experimental group B was significantly difference(p<.05). Conclusion : In conclusion, calf-raise exercise on unstable support surface was more efficient than stable support surface for increasing thickness of gastrocnemius.