The purpose of this study was to investigate the effects of knee joint muscle fatigue and overweight on shock absorption during single-leg landing of adult women. Written informed consent forms, which were approved by the human subject research and review committee at Dong-A University, were provided to all subjects. The subjects who participated in this study were divided into 2 groups: a normal weight group and an overweight group, consisting of 15 young women each. Both the normal weight group and the overweight group showed that use soft landing and ankle dominant strategy. The peak vertical ground reaction force, the knee joint absorption power, and eccentric work done, as the increase of knee joint muscle fatigue level, showed a decrease. And the hip joint absorption power and eccentric work done, as the increase of weight, was less than the overweight group showed the normal weight group. In conclusion, the accumulation of the knee joint muscle fatigue and the increase of body weight may lead to an increased risk of injury during landing.