The flexural behavior test of UHPC segmental box girder which has 160 MPa compressive strength and 15.4 m length was carried out. The effect of steel fibers in combination with reinforcing bars on improving the ductile performance of UHPC box girder was evaluated by comparing the flexural behavior of the UHPC segmental box girders made by the two kinds of mixing portion. The test variables are volume fraction of steel fibers and the arrangement of reinforcing bars. The behavior of UHPC box girder BF2 composed of 1% volume fraction of steel fibers and longitudinal reinforcing bars in web and upper flange with stirrup showed the similar ductile behavior with the girder BF1 composed of 2% volume fraction without stirrup in elastic stress region. But BF1 had the better stiffness and showed the more ductile behavior in inelastic stress region. Segmental interfaces of UHPC box girder have not any crack and slide until the final flexural collapse load.