Nanoporous anodic aluminum oxide (AAO) templates are fabricated using an nodization method. The mean diameters of nanoporous anodic aluminum oxide templates are 100 nm and 200 nm by various processing parameters of the anodization method. A molded plastic thin film nanostructure is fabricated by nanoimprinting using the AAO template as a mold insert. The surface properties of the molded plastic thin film are discussed using various nanoimprinting process parameters. Contact angles of the molded plastic thin film with the nanostructure exceed those without the nanostructure. The molded plastic thin films with a nanostructure and a hydrophobic surface are formed, and their contact angles exceed $90^{circ}$. This study observes the behavior of osteoblast-like cells (MG63) cultured on nanostructure thin films, i.e., AAO, polylactic acid (PLA) and polycarbonate (PC). Cell growth behavior indicates that the AAO template with a 200 nm nanostructure is best. This study shows that cell adhesion and spreading are influenced by surface topography in the nanometer feature.