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Effect of 7,8-Dihydroxyflavone on In Vitro Maturation of Oocytes in Pigs
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  • Effect of 7,8-Dihydroxyflavone on In Vitro Maturation of Oocytes in Pigs
  • Effect of 7,8-Dihydroxyflavone on In Vitro Maturation of Oocytes in Pigs
저자명
Oh. Seung-Kyu,Malaweera. Don Buddika Oshadi,Ramachandra. Sisitha,Shin. Sang-Tae,Cho. Jong-Ki
간행물명
韓國受精卵移植學會誌
권/호정보
2014년|29권 1호|pp.67-71 (5 pages)
발행정보
한국수정란이식학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

In porcine embryo culture, one of reactive oxygen species (ROS) is harmful factors that are made during in vitro culture. To decrease the detrimental effect of ROS on embryo development, superoxide dismutase, catalase and glutathione peroxidase could be used in the embryo culture. Out of these antioxidants, 7,8-dihydroxyflavone (7,8-DHF) was reported its antioxidant effects to prevent the glutamine-triggered apoptosis. Therefore, this study was performed to investigate the most appropriate concentration of 7,8-DHF in porcine embryonic development. For that, 5 different concentration (0, 0.1, 0.5, 1, $2{mu}m$) of 7,8-DHF was supplemented in the porcine IVM media and then maturation and blastocyst formation rates were compared among 5 groups. In maturation rates of porcine oocytes, significant higher maturation rates was shown in the $1.0{mu}m$ group compared with another 4 groups ($83.3{pm}2.1$ vs. $80.7{pm}1.4$, $79.8{pm}1.4$, $78.3{pm}1.2$, $79.4{pm}1.6$), respectively (P<0.05). In the embryo culture, $1.0{mu}m$ group also showed the significant higher cleavage rates ($76.8{pm}3.1$ vs. $62.1{pm}5.0$, $65.7{pm}4.0$, $68.6{pm}3.7$, $64.6{pm}4.0%$) and blastocyst formation rates - ($39.6{pm}4.0%$ vs. $28.6{pm}3.3$, $31.1{pm}3.9$, $29.3{pm}2.5$, $39.6{pm}4.0$, $26.4{pm}3.2%$), respectively (P<0.05). There was no significant difference among 5 groups in the cell number of blastocyst (P<0.05). In conclusion, supplement of $1.0{mu}m$ of 7,8-DHF was effective to increase the porcine embryonic development competence as antioxidant to ROS.