Vibration testing is conducted for evaluate the fatigue resistance of responsible system over excitation situations and two kinds of vibration profiles, harmonic or random, are widely used in engineering fields. Harmonic excitation profile is adequate for the rotating machinery that is primarily exposed to the orderly excited force subjected for a rotating speed; Random profile is suitable for the non-stationary vibration input, that is a ground excitation for example. Recently, the sine on random(SOR) testing method was sometimes considered to represent the real excitation conditions since the measured response signals of a target system, expecially for moving mobility, shows usually a mixture of them. So, it is important to understand the accumulated fatigue damage over different excitation patterns, harmonic and/or random, to determine the efficient vibration profile of a target system. A uniaxial vibration testing with a notched simple beam was introduced to evaluate the fatigue damage for different excitation profiles and the best choice of vibration profile was concluded from those comparison of calculated fatigue damages.