This paper presents a method for hand segmentation using depth information, and adaptive threshold by means of histogram analysis and color clustering in HSV color model. We consider hand area as a nearer object to the camera than background on depth information. And the threshold of hand color is adaptively determined by clustering using the matching of color values on the input image with one of the regions of hue histogram. Experimental results demonstrate 95% accuracy rate. Thus, we confirmed that the proposed method is effective for hand segmentation in variations of hand color, scale, rotation, pose, different lightning conditions and any colored background.