Using only laser range finder to detect the obstacles in an environment that contains transparent obstacles can not guarantee autonomous mobile robot from collision problem. To solve this problem, a mobile robot using laser range finder must be used additional sensor device such as sonar sensor that can detect the transparent obstacle. In this paper, a method is addressed to deal with the problem to detect the transparent obstacles within environment only by using laser range finder for mobile robot. In case the recognized transparent obstacle, the proposed algorithm is to localize the transparent obstacle to extract and process the reflected noise. This algorithm ensures autonomous of mobile robot only using laser range finder. The effectiveness of the proposed algorithm is evaluated by the real mobile robot and real laser range finder experiments with three case studies.