This study designed to know subjective structure about clinical experience of adaptation of new nursing staffs in a general hospital and using Q-methodology. Using Q-methodology, 40 new nursing staffs sorted working a general hospital through personal interview. The statement positions on the sorting continuum were scored from +4(representing the highest level of agreement) through 0(at the neutral position on the continuum) through to -4(representing the highest level of disagreement). Factor analysis of the data was undertaken using PC QUANL program to determine if any patterns were discernible. The five types extracted all had eigenvalues greater than 1.0 and explained 62.38% of the variation in responses.: (1) New workload type, (2) Interest and responsibility type, (3) Improve the situation type, (4) Lack of knowledge and technology type, and (5) Excessive workload type. The significant differences found in the subjective structure of clinical experience of adaptation of new nursing staffs in ad general hospital. The findings of this study could contribute to the development of an effective programmes of education and personnel clinical practice strategies.