The seismic vulnerability of stone masonry buildings is studied on the basis of their fragility curves. In order to account for out-of-plane failure modes, normally disregarded in past studies, linear static Finite Element analysis in 3D of prototype regular buildings is performed using a nonlinear biaxial failure criterion for masonry. More than 1100 analyses are carried out, so as to cover the practical range of the most important parameters, namely the number of storeys, percentage of side length in exterior walls taken up by openings, wall thickness, plan dimensions and number of interior walls, type of floor and pier height-to-length ratio. Results are presented in the form of damage and fragility curves. The fragility curves correspond well to the damage observed in masonry buildings after strong earthquakes and are in good agreement with other fragility curves in the literature. They confirm what is already known, namely that buildings with stiff floors or higher percentage of load-bearing walls are less vulnerable, and that large openings, taller storeys, larger number of storeys, higher wall slenderness and higher ratio of clear height to horizontal length of walls increase the vulnerability, but show also by how much.