In computer vision research, binarization procedure is one of the most frequently used tools to discriminate target objects from background in grey level binary image. Fuzzy binarization is a reliable technique in environment with high uncertainty such as medical image analysis by setting the threshold as the average of minimum and maximum brightness with triangle type fuzzy membership function. However, this technique is also known as contrast sensitive method thus its discrimination power is not so great when the image has low contrast difference between objects and backgrounds and suffer from information loss as a result. Thus, in this paper, we propose a fuzzy binarization using ART2 algorithm to handle such low contrast image analysis. Proposed ART2 algorithm is applied to determine the medium point of membership function in the fuzzy binarization paradigm. The proposed methods shows low information loss rate in our experiment.