Because of high oil prices and emission gas problems, many governments tighten regulation of fuel economy and emission gas. For Passenger car, there are many researches for plug-in hybrid electric vehicles and they are being manufactured. On the other hand, there are few researches for plug-in hybrid electric bus that is heavy commercial vehicle. In this study, analysis of fuel economy for series plug-in hybrid electric bus according to engine operation strategy based on simulation is conducted. Forward simulator is developed using Autonomie. Engine operation strategies consist on constant engine operation strategy and engine on/off operation strategy. Considering the engine operation strategy, results of vehicle speed, engine operating points and fuel economy are obtained and analyzed. As a result, engine on/off operation strategy has more advantage than constant engine operation strategy in terms of fuel economy.