A study on the static analysis of Timoshenko beams is presented. A beam element is developed by using isogeometric approach based on Timoshenko beam theory which allows the transverse shear deformation. The identification of transverse shear locking is conducted by three refinement schemes such as h-, p- and k-refinement and compared to other reference solutions. From numerical examples, the present beam element does not produce any shear locking in very thin beam situations even with full Gauss integration rule. Finally, the benchmark tests described in this study is provided as future reference solutions for Timoshenko beam problems based on isogeometric approach.