This paper investigates the relationship between acoustic characteristics and voice handicap index for 29 males with esophageal speakers. Acoustic characteristics were measured by using a sustained vowel /a/ three times. The stable vocalization for 2 seconds was analyzed by MDVP program. Specifically, relationships between four VHI scores (total, functional, physical, and emotional) and three acoustic characteristics (jitter, shimmer, and NHR) were investigated using the Pearson correlation coefficient. As results, we found no relationship between NHR and VHI scores. However, both jitter and shimmer had statistically significant correlations with all four VHI scores. This research will contribute to establishing a baseline related to speech characteristics in voice rehabilitation with esophageal speakers. Further research could be done to examine the overall quality of life survey, which is widely used as a subjective measure about voice for patients with esophageal speakers.