Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED) and causes a considerable economic loss in swine industry. In this study, the virucidal efficacy of the disinfectant composed to n-alkyl-dimethyl-benzyl-ammonium chloride (n-ADBAC) was investigated against PEDV. A virucidal efficacy was determined with the viability of PEDV contacted with the disinfectant in Vero cells. The disinfectant and PEDV were reacted on the hard water (HW) or organic matter suspension (OM) condition. On HW condition, PEDV was inactivated with 50 fold dilutions of the disinfectant. When the antiviral effect on OM condition was evaluated, the antiviral activity of the disinfectant showed on 10 fold dilutions against PEDV. As the disinfectant possesses the virucidal efficacy against PEDV, the disinfectant solution can be used to limit the spread of animal viral diseases.