Objective : The purpose of this study is to offer a new approach to diagnostic X-ray in perspective of Chuna manual medicine for clinical application. Methods : Characteristics of each malposition in X-ray were analyzed comprehensively, based on the listing system. By verifying these results, find out the methods of X-ray diagnosis according to the each malposition. Results : 1. Vertebral malposition can be explained by alignment and relative position of vertebral body in the X-ray. To obtain more accurate estimation of subluxation, features of other structures should be considered, such as spinous process, intervertebral foramen and disc space. 2. Pelvic malposition can be determined by relative location of anterior superior iliac spine (ASIS) and posterior superior iliac spine (PSIS) in the X-ray. Also other pelvic parameters should be utilized to make a diagnosis of sacral malposition. Conclusions : Diagnostic X-ray should be applied to many clinicians for reasonable Chuna manual medicine application. And further studies are needed to use the diagnostic X-ray in the perspective of Chuna manual medicine.