Marine-derived microbes have yielded a variety of metabolites so far. In the course of the project to find metabolites from marine microbes, an isolate of Penicillium glabrum (SF 5317) was selected for chemical investigation. A large scale culture of this strain in PDA media was extracted with an organic solvent and the extract was subjected to a serious of chromatography, which led to six metabolites. Their chemical structures were elucidated as sulochrin (1), questin (2), questinol (3), ergosterol (4), 3-methyldesmethoxyyangonin (5), and hydroxysulochrin (6) on the basis of spectroscopic data. To the best of our knowledge, this is the second isolation of compounds 5 and 6 in nature and especially 6 is isolated from Penicillium sp. for the first time.