Use of plant based remedies for the prevention and treatment of diabetic complications over conventional therapies have received much emphasis in recent years. Present study was performed to evaluate the protective effect of composite extract (CE) of leaves of Aegle marmelos, Ocimum sanctum, Murraya koenigii and Azadirachta indica on biochemical alterations in alloxan (ALX) induced diabetic wistar rats. Diabetes was induced in rats by a single administration of ALX (150 mg/kg intraperitoneal (i.p) and CE at three different doses (25, 50 and 100 mg/kg/bw/day) were administrated orally in three group of diabetic rats for 35 days. Another group of same number of diabetic rats were administrated by insulin (6 unit/kg/bw/day) by subcutaneous injection for 35 days and used as standard. Results showed that oral administration of CE significantly protected the biochemical impairments in diabetic rats as evidenced by restoration of glutathione depletion, ascorbic acid level, plasma antioxidant values and inhibition of lipid peroxidation. The outcome of the study suggests that CE of A. marmelos, O. sanctum, M. koenigii and A. indica leaves mimics insulin and passes capacity to ameliorate the hyperglycemia induced cellular complications thus may control the development and progression of diabetes.