To improve oral hygiene management, the effects of toothbrush training on general oral hygiene based on the simplified oral hygiene index(S-OHI) and degree of knowledge about toothbrushing were examined. Pre-and post-training changes in the S-OHI (lower score = better oral health status) showed a significant decrease in score in all variables (gender, age, marital status, occupation, and level of education).Pre- and post-training changes in the degree of knowledge about toothbrushing showed a significant increase. Negative correlations between the S-OHI, oral health status, and degree of knowledge about toothbrushing and the S-OHI and the Decayed, Missing, Filled (DMF) index (the better the oral cavity environment, the lower the DMF index) were observed. Positive effects of repeated toothbrush training on the maintenance of healthy oral cavity environment and continuous oral health management were observed, as reflected by the correct toothbrushing-related knowledge and skills.